Carbons 3 and 4 of glucose, if metabolized via glycolysis and the citric acid cycle, would be most rapidly lost as CO2.
Glycolysis is the process which involves breakdown of glucose within the cytoplasm of a cell thus producing pyruvate.
During aerobic conditions, pyruvate can diffuse into mitochondria, where it enters the citric acid cycle and forms reducing equivalents in the form of NADH and FADH2.
The Citric acid cycle involves a series of enzyme catalyzed reactions taking place in the mitochondrial matrix, during which acetyl-CoA is oxidized to form carbon dioxide and coenzymes are reduced leading to the generation of ATP in the electron transport chain.
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