DNA and RNA base sequences, by convention, are written in the 5' to 3' direction.
A consequence of the structure of nucleotides is that a DNA and RNA (polynucleotide chains) has directionality, which means it has two ends that are different from each other.
At the 5’ end, or beginning, of the chain, the 5’ phosphate group of the first nucleotide in the chain sticks out.
At the other end, called the 3’ end, the 3’ hydroxyl of the last nucleotide added to the chain is exposed.
DNA sequences are usually written in the 5' to 3' direction, meaning that the nucleotide at the 5' end comes first and the nucleotide at the 3' end comes last.
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