Answer:
Helicase untwists the double helix at the replication fork Topoisomerase helps releive the strain caused by DNA untwisting. It breaks, swivels and rejoins DNA strands.
Single-stranded binding proteins bind the unpaired DNA strands, keeping them from re-pairing.
Primase starts a complementary RNA chain using parental DNA strand as a template
DNA polymerase III starts adding DNA nucleotides to the 3' end of the existing RNA chain.
Elongation of leading strands occurs continuosly and elongation of lagging strands occurs via generation of Okazaki fragments. Both are synthesized in 5' to 3' direction.
DNA ligase joins Okazaki fragmments together
DNA polymerase I removes the RNA primers and replaces them with DNA nucleotides
Explanation:
Helicases are enzymes that form replication fork by disrupting the hydrogen bonds between base pairs in order to separate the strands into a Y shape. The replication fork is bi-directional: one strand is oriented in the 3' to 5' direction and it is called leading strand while the other is oriented 5' to 3' and it is called lagging strand.
DNA polymerase is the main enzyme for the replication of DNA. It requires primer in order to start replication. DNA plymerase synthesizes the new strands by adding nucleotides that complement each (template) strand .